Molecular Epidemiology of ESBL Genes and Multi-Drug Resistance in Diarrheagenic Escherichia coli strains Isolated from Adults in Iran

Authors

  • Abbas Doosti Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • Meysam Sarshar Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
  • Mohammad Kargar Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
  • Pejman Abbasi Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Sadegh Ghorbani-Dalini Department of Microbiology, Jahrom Branch, Young Researcher’s & Elit Club, Islamic Azad University, Jahrom, Iran
Abstract:

Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum β-lactamases (ESBLs). Clear identification of risk factors for ESBL-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs pattern of diarrhogenic Escherichia coli (E. coli) strains isolated from adult patients. In the present study, Diarrheogenic E. coli strains were isolated from 54 patients from the University of Medical Sciences hospitals in Shiraz. Antimicrobial susceptibility testing was done by disk diffusion method by CLSI criteria. The presence of blaTEM, blaSHV and blaCTX-M genes was investigated by PCR using designated primers. The prevalence of ESBLs-producer E. coli strains was 12.96%. Antimicrobial resistance testing showed a high resistance to cefexime, trimethoprim-sulfamethoxazole, ampicillin and penicillin. Overall, β-lactamase genes were identified in 52 (96.30%) isolates which were identified as 45 (83.33%) blaTEM, 17 (31.48%) blaSHV and 11 (20.37%) blaCTX-M. ESBL-producer E. coli is very prevalent in Diarrheogenic strains isolated from adult patients. Also, this study clearly showed that the blaTEM gene for ESBL-producer E. coli was widespread in Iran.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

molecular epidemiology of esbl genes and multi-drug resistance in diarrheagenic escherichia coli strains isolated from adults in iran

resistance to oxyimino cephalosporins antibiotics in enterobacteriaceae is primarily done by the extended spectrum β-lactamases (esbls). clear identification of risk factors for esbl-producing infections is necessary. therefore, efficient strategies can be developed to decrease outbreak of these infections. the aim of this study was to determine the antibacterial susceptibility and esbls patter...

full text

Molecular Epidemiology of ESBL Genes and Multi-Drug Resistance in Diarrheagenic Escherichia Coli Strains Isolated from Adults in Iran

Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum β-lactamases (ESBLs). Clear identification of risk factors for ESBLs-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs patte...

full text

Molecular Monitoring of Fosfomycin Resistance in Escherichia coli Strains Isolated from Patients with Urinary Catheters in north-east of Iran

Introduction: Urinary tract infection is a common nosocomial infection that has recently become difficult to treat because of the increased emergence of multi-drug resistant strains. This study aims to determine the minimum inhibitory concentration, and molecular pattern of resistance to fosfomycin in Escherichia coli isolates originated from patients hospitalized with urinary tract infection i...

full text

Molecular detection and antimicrobial resistance of diarrheagenic Escherichia coli strains isolated from diarrheal cases.

OBJECTIVE To identify and classify Iranian isolates of diarrheagenic Escherichia coli (E. coli) on the basis of presence of virulence genes and to determine antibiotic susceptibility of isolated strains. METHODS The current cross-sectional study was conducted in 2005 at the Pasteur Institute, Tehran, Iran. One hundred and ninety-three diarrheagenic E. coli isolated from diarrheal patients in ...

full text

Antibiotic Resistance in Diarrheagenic Escherichia coli Isolated from Broiler Chickens in Pakistan

Background: Diarrheagenic Escherichia coli (DEC) strains are predominant cause of gastrointestinal tract illnesses. The main objective of the study was to determine antibiotic resistance in various types of DEC isolated from chicken broilers farmed in Pakistan. Methods: A total of 200 feces and 200 meat samples from broiler chickens were collected from the slaughtering shops in Southern Punjab...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 14  issue 4

pages  1257- 1262

publication date 2015-10-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023